
Bioremediation is the natural process of degrading organic materials to carbon dioxide, water and various ions. It is literally as old as life on earth. Microbes that exist naturally in all soil and water produce enzymes which breakdown hydrocarbons into smaller, less toxic materials.
Using modern biotechnology we have found ways to accelerate and improve the effectiveness of bioremediation. Naturally occurring microbes have been selectively adapted for their ability to digest specific hydrocarbon contaminants such as oil and gasoline. The Microbes are combined with nutrients, pH stabilizers, oxygen and surfactants. The result is a product which when applied to contaminated soil or water, optimizes the environment for bioremediation to take place. The remediation process is accelerated dramatically. The contamination is mineralized and the environmental hazard is reduced or eliminated.
In many cases, petroleum encountered in a site investigation has been chemically altered due to environmental exposure, and its property change - sometimes radically - resulting in a gas chromatographic trace with little resemblance to the unaltered product. To the untrained eye, these altered GC traces can be misinterpreted, leading to the wrong conclusions about source, transport and fate of petroleum in the study area.
Occasionally, samples collected from a site contain petroleum products or related wastes that have remained chemically intact since the time of release. Using well-established laboratory and gas chromatographic techniques, a laboratory analyst with reasonable experience can usually identify the petroleum present in such samples.
The three most commonly encountered weathering pathways that affect fugitive petroleum are microbial degradation, evaporation and solubilization.
Microbial degradation. There are indigenous microbial populations in most environmental media capable of degrading petroleum given favorable conditions such as availability of oxygen, water, and nutrients. Although almost all of the major chemical classes of petroleum are biodegradable, the normal alkanes are most susceptible to this weathering pathway, and are usually the first compounds to show evidence of degradation following a release.
Evaporation. Selective losses of low molecular weight compounds - molecular weight of about 150 a.m.u. and less due to evaporation can be a significant part of the weathering process. Obviously, the lighter the spilled product is gasoline as opposed to crude, for example, the more likely that evaporative losses will constitute a significant portion of the overall weathering.
Solubilization. Most hydrocarbons that make up petroleum have low solubility in water, however certain compounds, notably the lower molecular weight aromatics and some low molecular weight aliphatics -have reasonable was solubility. For example, the monoaromatic compounds, principally benzene, ethylbenzene, toluene and the xylenes (BTEX), lesser alkylated benzenes (< C4), and naphthalene and its C1- analogues have notable solubilities, such as solubility of benzene at 1780 mg/L to 2-methylnaphthalene - 25 mg/L.
Armed with such knowledge, the site investigator can confidently identify and track fugitive petroleum at a study site. If faced with a weathered product that must be distinguished from other petroleum sources of like or different composition, it also allows the investigator to tailor further, more sophisticated chemical analyses and interpret those results in light of the weathering state of the in-place petroleum.
1.1 Chemical Used for Bioremediation
Surfactant can enhance bioremediation performance. Surfactants can act in two ways which are increase solubility and lower the interfacial tension by reducing the capillary forces.
(a) BioSurfactant - BioSolve
BioSolve is water based and biodegradable that will accelerate the bioremediation by:
i) Increasing the speed of carbon substrate exposure to the microorganisms and the dispersion of the micro-organism’s’ enzymes
ii) Desorbing and dispersing the contaminate into a more acceptable environment for micro-organisms ( aqueous phase)
iii) Encapsulating VOC’s into the water to prevent volatilisation
(b) Micro Nutrient - Desludge
Micro nutrient is an environmentally, bio degradable, non-toxic, non-hazardous product. It designed to be used with naturally occurring bacteria or with an enhanced bacteria package. It can keep the bacteria aerobic for longer time. Will consume more contaminated oil and faster. Does not contain enzymes, formaldehyde or chlorine; is not a biocide as well as practical and economical.
1.2 Application of BioSolve
l Soil remediation
- Enhance the microbe’s ability to metabolize the contaminated
l Vapor Suppression
- Encapsulates the source of the vapor rather than temporarily blanketing it like foam
l Bioremediation
- Produced excellent results when used in conjunction with nutrients
- Shorter time frame and greatly reduced overall costs to remediate
l Water Treatment
- Proven effective in accelerating the degradation levels within legal discharge limits.
l In Storage Tank
- Proven effective in eliminating or reducing VOC vapors
l Spill Clean –Up
- Emulsifies and encapsulates the contaminants almost instantly
- Helping to eliminate the danger
l In Sewers
- Successfully utilized by Emergency Response Teams in numerous instances of volatile hydrocarbon discharges into sewer systems.
- Immediately helps reduce or eliminate the vapor release and the danger of fire or explosion.
1.3 Some others chemical groups that Biosurfactant can be used (other than
Hydrocarbon)
1. Glycol Ether 7. Alcohols
2. Glycol Ether Acetates (*) 8. Aliphatic Solvents
3. Jeffamine Ployoxypropleneamines(*) 9. Aromatic Solvents
4. Jeffcool Industrial Coolants (*) 10. Esters
5. Jeffox Polyethylene Glycols (*) 11. Glycols
6. Keytones
Note: (*) Commercial named group
1.4 Uses of BioSolve
Biosurfactant has a wide range of users and uses such as Environmental Clean-Up Companies, Petroleum Tank Farms, Oil Refineries, Fire Department Hazmat Teams, Water Treatments Facilities, Manufacturers, Utility Companies, Airports, Military Shipyards, Marinas, Drilling Platform and many more.
Solve can enhance the microbe’s ability to metabolise the contaminate by stripping the hydrocarbon off the soil particles and emulsifying it into the pore space
Hydrocarbon that has been stripped off from soil particles
BioSolve encapsulates the contaminate in water so that it can’t vaporize and to allow bacteria quickly metabolise the contaminate
Microorganisms digest hydrocarbon and convert it to carbon dioxide and water
Oil
Microbe
CO2 + H2O
CO2 + H2O
Microorganisms eat hydrocarbon or other organic contaminant
Microorganisms give off carbon dioxide and water
1.5 BioSolve Overview
BioSolve is a unique blend of biodegradable, water based surfactants, wetting agents and emulsifier developed after years formulation. Our team was developed for, and has been utilized by the environmental clean up for over 5 years as an effective solution to enhancing many technologies for contaminant mitigation. These technology include bioremediation, VOC vapor & odor suppression, tank entry, workover turnarounds, UST washouts, emergency response, spill mitigation, soil washing and sludge separation.
BioSolve can be stored for long-term storage, which is more than 10 years with no deterioration of quality in unopened container. It is effective with hard, soft, brackish or salt waters and also not affected by freezing, simply thaw and stir.
BioSolve is the result of tests on hundreds of formulations. It is a uniquely balanced blend of water based nonionic with a slight amount of anionic surfactant. BioSolve does not contain cationic surfactants nor does it contain phosphates, nitrates or D-Limonene.
So far there is no one record highlighted the side effect of using our biosolve to the system treated and environment.